What was the purpose of the VSV-G pseudotyped lentivirus in the cellular infections?

The VSV-G protein has been the most popular for pseudotyping the envelope proteins of lentiviral vectors. VSV-G binds LDL-Receptor family members, allowing these viruses to infect a wide range of cell types of many distinct host species, including rodent and human cells [15].

What is VSV-G lentivirus?

Abstract. Viral fusion proteins are essential for enveloped virus infection. These proteins mediate fusion between the virus envelope and host cellular membrane to release the viral genome into cells. Vesicular stomatitis virus G (VSV G) protein is a typical type III viral fusion protein.

What does VSV-G bind?

LDL-R has been demonstrated to be the major entry port of VSV and lentivirus pseudotyped by VSV G14. Here, we demonstrate that VSV G is able to bind two CR domains of the LDL-R with similar affinities.

Is VSV a lentivirus?

The VSV-G envelope protein is commonly used in lentiviral particle production because it confers broad tropism over a range of species and cell types. For more information, see the Cronin, et al. article on different envelopes and their tropism.

Why is VSV used?

Recombinant vesicular stomatitis virus (VSV) vectors are widely used for a variety of applications including studying mechanisms of viral entry into host cells, identification of cellular receptors utilized by viruses for cell entry, screening of viral entry inhibitors and vaccine development research.

Why is lentivirus used for transduction?

Lentiviral transduction is an efficient method for the delivery of transgenes to mammalian cells and unifies the ease of use and speed of transient transfection with the robust expression of stable cell lines.

What receptor does VSV use?

LDL receptor
LDL receptor and its family members serve as the cellular receptors for vesicular stomatitis virus. Protein transfer into human cells by VSV-G-induced nanovesicles.

What is lentivirus transduction?

How does VSV enter cell?

For the vesicular stomatitis virus (VSV), the receptor is ubiquitous, but unknown, and virus enters cells via clathrin-mediated endocytosis, with fusion occurring with endosomes at a pH of approximately 5.8.