What is a molecular mass ruler?

Bio-Rad precision molecular mass rulers (DNA ladders) allow accurate DNA quantitation in gels, making them ideal for densitometry or image analysis. The EZ Load precision molecular mass ruler is ready to load.

Why do we use the precision molecular mass ruler in an agarose gel?

Use our Precision Molecular Mass Ruler for accurate quantitation of the amount of DNA in a sample band after gel electrophoresis. EZ Load Molecular Rulers are pre-blended with sample loading buffer and ready to use; store at 4°C. Recommended gels: Standard agarose ≥1%

What is DNA ruler?

Introduction. A DNA ladder is a solution of DNA molecules of different lengths used in agarose or acrylamide gel electrophoresis. It is applied as a reference to estimate the size of unknown DNA molecules that were separated based on their mobility in an electrical field through the gel.

What is DNA size standard?

Typical size standards are made up of DNA or RNA fragments in variable length in the range of 10bp to 1000bp (base pair) increments. One universally used DNA ladder measures up to 1 kilobase pair (1Kb) and contains 1-10 Kb fragments.

What is the purpose of using a DNA size marker?

Essentially, a typical DNA size marker comprises from discrete DNA fragments of specific size ranging from tens to thousands of base pairs (bp) where application of these to agarose gel provides a visual reference to help to estimate size of unknown DNA molecules (2).

What is 1Kb DNA ladder?

1 kb DNA Ladder allows for determining the size of double-stranded DNA from 250 – 10,000 base pairs (bp). The ladder consists of 13 double-stranded, blunt-end fragments, of sizes 250, 500, 750, 1000, 1500, 2000, 2500, 3000, 4000, 5000, 6000, 8000, and 10,000 bp (see Figure 1).

What is DNA size marker?

DNA and RNA size markers contain a mixture of DNA (or RNA) fragments of known length, making them suitable for estimating the fragment length of concurrently run samples. Markers and ladders stain well with ethidium bromide and other common nucleic acid stains for visualization after gel electrophoresis.

How do you determine the size of DNA fragments from electrophoresis?

Using electrophoresis, we can see how many different DNA fragments are present in a sample and how large they are relative to one another. We can also determine the absolute size of a piece of DNA by examining it next to a standard “yardstick” made up of DNA fragments of known sizes.

How much PCR should I load on gel?

Template gel A volume of 2 μl of purified PCR product should be loaded on the gel. After electrophoresis, bands should be easily visible. If bands are faint, the amount of template for sequencing can be increased.