What does RPMI 1640 stand for?

Roswell Park Memorial Institute
RPMI-1640 was developed by Moore et. al. at Roswell Park Memorial Institute, hence the acronym RPMI.

What is RPMI 1640 where and for what purpose this chemical is used?

RPMI 1640 has traditionally been used for the serum-free expansion of human lymphoid cells. RPMI 1640 uses a bicarbonate buffering system and differs from most mammalian cell culture media in its typical pH 8 formulation.

Can I use expired RPMI?

You might need to supplement with some additional ingredients which might have degraded over time. RPMI 1640 shelf life is 18 months. I have used expired RPMI 1640 just for cell maintenance. Most likely is L-glutamine degrades.

Does RPMI 1640 contain HEPES?

RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas….This RPMI 1640 is modified as follows:

With Without
• Phenol Red • L-glutamine
• HEPES

What does RPMI media contain?

RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle’s Minimal Essential Medium or Dulbecco’s Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors.

What is RPMI in cell culture?

RPMI, also known as RPMI 1640 or Roswell Park Memorial Institute Medium, was first described by Moore (1967) in successfully culturing and expanding human lymphoid cells. RPMI contains 19 essential and non-essential amino acids including glycine, 11 vitamins, 6 salts, and sodium bicarbonate.

What kind of cell types is RPMI medium used for?

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.

How long is RPMI good for?

RPMI 1640 has a shelf life of 18-24 months, depending on formulation.

Does RPMI need to be refrigerated?

Samples in RPMI (except CSF) that are expected to be shipped within 12 hours are stable at room temperature–beyond 12 hours should be refrigerated. They should be shipped on a cool pack, but never frozen.

Does RPMI need HEPES?

RPMI is manufactured as enriched medium with or without L-glutamine with a stable L-glutamine substitute, with or without HEPES. RPMI 1640 requires supplementation with serum, typically 10% Fetal Bovine Serum (FBS), since this medium does not contain proteins, lipids, or growth factors.

How do I make my RPMI 1640 medium?

RPMI 1640 (11-100-1) – 1 liter

  1. Prepare 900ml of distilled water in clean glass beaker.
  2. Add the 1-liter powder to the water and stir gently.
  3. Add 2 gram Sodium Bicarbonate (or 26.67 ml of 7.5% Sodium Bicarbonate Solution).
  4. Adjust pH to 0.2-0.3 units below the required pH using 1N HCl or 1N NaOH.