How is DPPH scavenging activity calculated?

The percentage of DPPH scavenging effect was calculated by following equation. DPPH scavenging effect (%)/% Inhibition=A0-A1/A0 × 100 Where A0=The absorbance of control. A1=The absorbance of sample. As DPPH was soluble in methanol, it was taken up as organic phase.

What is ABTS radical scavenging activity?

The ABTS assay measures the relative ability of antioxidants to scavenge the ABTS generated in aqueous phase, as compared with a Trolox (water soluble vitamin E analogue) standard. The ABTS is generated by reacting with a strong oxidizing agent (eg, potassium permanganate or potassium persulfate) with the ABTS salt.

What is the role of DPPH in antioxidant activity?

The DPPH assay is used to predict antioxidant activities by mechanism in which antioxidants act to inhibit lipid oxidation, so scavenging of DPPH radical and therefore determinate free radical scavenging capacity. The method is widely used due to relatively short time required for the analysis.

What is IC 50 in DPPH?

The IC50 value is a parameter widely used to measure the antioxidant activity of test samples. It is calculated as the concentration of antioxidants needed to decrease the initial DPPH concentration by 50% [23]. Thus, the lower IC50 value the higher antioxidant activity.

How do you make a 0.1 mM DPPH?

1 M of DPPH = 394.32 gram of DPPH in 1 liter of solution (make the volume up to 1-liter level after dissolving the DPPH in less volume of methanol). 1 mM of DPPH must contain 0.394 grams in a 1-liter solution. 0.1 mM DPPH must contain 0.0394 gram of DPPH in 1 liter of solution.

What is the difference between DPPH and ABTS?

Differences between DPPH and ABTS radical scavenging activities can be ascribed to reaction media. DPPH assay is conventionally conducted under 50% ethanol /water, whilst ABTS assay is carry out in aqueous conditions. Besides, flavonoids solubility in both media should be taken in consideration.

What is the control for DPPH?

For DPPH assay methanol is used as blank where as DPPH + methanol is used as experimental control.

How antioxidants scavenge free radicals?

Antioxidants are compounds that can scavenge free radicals by interrupting radical chain reactions, or even prevent the reactive oxidants from being formed in the first place (Huang et al. 2005). Adverse effects of free radicals on aquatic organisms have been reported elsewhere.

What is DPPH free radical scavenging?

α, α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging method offers the first approach for evaluating the antioxidant potential of a compound, an extract or other biological sources. This is the simplest method, wherein the prospective compound or extract is mixed with DPPH solution and absorbance is recorded after a defined period.

Can scavenging free radicals be explained by substitution in aromatic rings?

However, even if these remarks could fit to some compounds as for isidiophorin, pulmonarianin, stictic acid, it cannot explain the good activity observed for lecanoric acid, 1′-chloropannarin and pannarin. In fact, the effects of substituents in the aromatic ring may cause different behaviour in the scavenging free radical activity.

Is plum (Prunus domestica) a free radical scavenger?

Free radical scavenging capacity and antioxidant activity of methanolic and ethanolic extracts of plum (Prunus domestica L.) in both fresh and dried samples Amin Morabbi Najafabad1and Rashid Jamei1,* Amin Morabbi Najafabad 1Department of Biology, Faculty of Science, Urmia University, Urmia, I. R. Iran Find articles by Amin Morabbi Najafabad

What is the scavenging effect of DPPH in methanol?

DPPH scavenging effect (%)/% Inhibition=A0-A1/A0× 100 Where A0=The absorbance of control. A1=The absorbance of sample. Results Optimization of mobile phase As DPPH was soluble in methanol, it was taken up as organic phase.