What stain is used for adipocytes?

Oil red O is a dye that strongly stains lipids. It is inexpensive and nonhazardous, and staining can be performed within a few hours. Oil red O has been used to discern preadipocytes from adipocytes, i.e. qualitative analysis, for more than 40 years.

Are fibroblast cells differentiated?

assessed a number of histochemical markers, but the derived cells differed from foreskin fibroblasts, which are a specific type of differentiated cell.

What do fibroblasts differentiate into?

As immature cells, fibroblasts can differentiate into other connective tissue cells, such as chondroblasts and osteoblasts. Human Dermal Fibroblasts (HDF, from Cell Applications) can also be converted into induced pluripotent stem cells (iPSC), a Nobel-prize awarded technology.

Is fibroblast a stromal cell?

Fibroblasts, known for a much longer time but still poorly characterized, are also considered to be a ubiquitous stromal element of almost all tissues and are believed to play a role in tissue homeostasis.

How does Oil Red O stain lipids?

Oil Red O is a lipid-soluble lysochrome (C26H24N4O) with a maximum absorption at 518 nm. This dye is used for the staining of neutral lipids (triglycerides and diacylglycerols), in addition to cholesterol esters, but does not bind to biological membranes.

What does Oil Red O measure?

BioVision’s Lipid (Oil Red O) Staining Kit allows selective detection of neutral lipids within cultured cells. The kit also includes Hematoxylin to stain nuclei. This kit provides enough reagents to stain two 96-well plates, two 6-well plates, or four 100 mm culture dishes.

Are fibroblasts pluripotent?

Dermal fibroblasts are also one of the cells types from which induced pluripotent stem cells (iPSCs) can be derived. Introduction of certain cellular factors (commonly OCT3/4, SOX2, KLF4, and C-MYC) converts human fibroblasts to cells with pluripotent potential, termed iPSCs.

What causes adipocyte differentiation?

Insulin activates its receptor to induce uptake of glucose and free fatty acids by adipocytes. Glucose and free fatty acids are building materials in the biosynthesis of triglycerides, which is stored in the cytoplasm of adipocytes and often used as a marker of adipocyte differentiation.