How do you perform a filaria blood smear?

The microfilariae that cause lymphatic filariasis circulate in the blood at night (called nocturnal periodicity). Blood collection should be done at night to coincide with the appearance of the microfilariae, and a thick smear should be made and stained with Giemsa or hematoxylin and eosin.

How do you prepare a blood smear for microscopy?

  1. Place clean glass slide on a flat surface. Add one small drop of blood to one end.
  2. Take another clean slide, and holding at an angle of about 45 deg, touch the blood with one end of the slide so the blood runs along the edge of the slide by capillary action.
  3. Make 2 smears, allow to air dry, and label clearly.

How blood smear is prepared?

Preparation. A blood smear is made by placing a drop of blood on one end of a slide, and using a spreader slide to disperse the blood over the slide’s length. The aim is to get a region, called a monolayer, where the cells are spaced far enough apart to be counted and differentiated.

How do you test for filariasis?

The diagnosis of filariasis requires examination of a blood smear for the presence of the larval round worm W. bancrofti or B. malayi. Since the number of parasites (parasitemia) in the blood is higher during the night, blood samples are best obtained at night.

How do you collect a blood sample for an exam?

Ask the patient to make a fist; avoid “pumping the fist.” Grasp the patient’s arm firmly using your thumb to draw the skin taut and anchor the vein. Swiftly insert the needle through the skin into the lumen of the vein. The needle should form a 15-30 degree angle with the arm surface. Avoid excess probing.

What is filaria antigen test?

Test Usage: This assay helps in the detection of Microfilaria in the peripheral blood in both lymphatic and non-lymphatic filariasis.

How do you test blood for microfilaria?

Usually, a microfilariae blood test is conducted at night to coincide with the appearance of microfilariae. The microfilaria test procedure requires a blood sample either from the finger prick, earlobe or venous blood. There are a number of tests to identify the presence of microfilariae in one’s body.

What is the basic technique to collect blood?

Venipuncture is the most common way to collect blood from adult patients. Collection takes place from a superficial vein in the upper limb, generally the median cubital vein; this vein is close to the skin and doesn’t have many large nerves positioned close by. This reduces pain and discomfort for the patient.