What does WGA binds to?

Wheat germ agglutinin (WGA) is a lectin that protects wheat (Triticum) from insects, yeast and bacteria. An agglutinin protein, it binds to N-acetyl-D-glucosamine and Sialic acid. Succinylated WGA is selective for β-GlcNAc, making it a useful tool for detecting O-GlcNAc.

Where does WGA bind?

WGA, primarily, binds to N-acetyl-D-glucosamine (GlcNAc) and is also reported to interact with sialic-acid-containing glycoconjugates and oligosaccharides.

What is WGA staining?

Wheat germ agglutinin (WGA), which binds to glycoproteins of the cell membrane, is routinely used for the staining of skeletal and cardiac sarcolemma to determine cross sectional area or myocyte density. In this context we observed that WGA also labeled fibrotic tissue in cardiac sections after myocardial infarction.

What color is wheat germ agglutinin?

A wheat germ agglutinin (WGA) lectin labeled with Biotium’s CF® Dyes or HRP….Product Attributes.

Probe cellular localization Membrane/cell surface
Colors Blue, Green, Orange, Red, Far-red, Near-infrared

What type of biomaterial are lectins like WGA?

3.3. The lectin is a homodimer composed of subunits of Mr = 23 600 Da which dissociates into monomers at acid pH. It is a pure, metal-free protein devoid of carbohydrate residues, which is isolated as a mixture of four isolectins differing in electrophoretic mobility [116,117].

What type of biomaterial is WGA?

WGA is a protein found abundantly in wheat kernels. It has a high binding affinity for N-acetyl-glucosamine (GlcNAc), one of the essential components of PG glycan strands, and thus can be used to probe PG specifically.

Where is WGA found?

wheat kernels
WGA is a protein found abundantly in wheat kernels. It has a high binding affinity for N-acetyl-glucosamine (GlcNAc), one of the essential components of PG glycan strands, and thus can be used to probe PG specifically.