What is the purpose of Murashige and Skoog medium?

Murashige and Skoog medium (or MSO or MS0 (MS-zero)) is a plant growth medium used in the laboratories for cultivation of plant cell culture. MS medium was originally formulated by Murashige and Skoog in 1962 to optimize tobacco callus bioassay system for facilitating the study of cytokinins.

How do you use Murashige and Skoog medium?

Reconstitute medium by adding required quantity of powder in two-third of total volume with constant, gentle stirring till the medium gets completely dissolved. Add heat stable supplements prior to autoclaving. Make up the final volume with distilled water. Adjust the pH of the medium to 5.75 ± 0.5 using 1N NaOH/ HCl.

What is the composition of Murashige and Skoog medium?

MS1 for plant tissue culture is suitable for growing callus and different plant organs. Composition mg/L: NH4NO3 1650, KNO3 1900, CaCl2. 2H2O 440, MgSO4.

How do you make vitamin stock solution?

Vitamin Stock (100x) Take a 100 ml beaker and add 50 ml double-distilled water in it. Weigh the vitamins given in the table below and dissolve it completely in the water. Transfer the solution to a volumetric flask of 100 ml and makeup to the final volume.

What is plant callus?

callus, In botany, soft tissue that forms over a wounded or cut plant surface, leading to healing. A callus arises from cells of the cambium. When a callus forms, some of its cells may organize into growing points, some of which in turn give rise to roots while others produce stems and leaves.

Which vitamin is used in MS media?

Major Components of MS media It is categorized into two groups: Macronutrients (Calcium, magnesium, nitrogen) and micronutrients (copper, iron, and zinc). Organic nutrient: It mainly includes vitamins and amino acids, required for the growth and differentiation of the cultures.

What are the ingredients of MS medium?

Ingredients

  • Ammonium nitrate (NH4NO3) 1650 mg/l.
  • Calcium chloride (CaCl2 · 2H2O) 440 mg/l.
  • Magnesium sulfate (MgSO4 · 7H2O) 370 mg/l.
  • Monopotassium phosphate (KH2PO4) 170 mg/l.
  • Potassium nitrate (KNO3) 1900 mg/l.

What is the pH of MS media?

The pH of the medium is adjusted to 5.8±0.2 using dilute NaOH or HCl and a suitable gelling agent is added when a semisolid matrix is desired. Agar (8–10 g/l) and the gellan gum known as Phytagel® or Gelrite® (2–3 g/l) are two of the most common gelling agents used for plant tissue culture media.