Do HEK293 cells express TLR4?

Because HEK293 cells do not express endogenous MD-2, HEK293 cells expressing TLR4 responded to LPS/MD-2 but not to LPS alone (25), suggesting that TLR4 was expressed on the cell surface and bound to the LPS/MD-2 complex. We established HEK293 cells stably expressing mouse TLR4 fused with GFP.

What Ishek 293?

Human embryonic kidney 293 (HEK293, HEK-293, or HEK) cells are one of the most common cell lines used for research purposes, second only to HeLa cells. Their popularity is due to several properties such as easy maintenance, robustness, and ease of transfection.

What are HEK blue cells?

HEK-Blue™ Detection is a cell culture medium that allows the detection of SEAP (Secreted embryonic alkaline phosphatase) as the reporter protein is secreted by the cells. HEK-Blue™ Detection contains all the nutrients necessary for cell growth and a specific SEAP color substrate.

What is SEAP in biology?

Secreted embryonic alkaline phosphatase (SEAP) is a reporter widely used to study promoter activity or gene expression. It is a truncated form of human placental alkaline phosphatase (PLAP) by deletion of the GPI anchor.

What is HEK293 used for?

The human embryonic kidney (HEK) 293 cell line and its derivatives are used in experiments ranging from signal transduction and protein interaction studies over viral packaging to rapid small-scale protein expression and biopharmaceutical production.

What are 293 cells?

293 [HEK-293] is a cell line exhibiting epithelial morphology that was isolated from the kidney of a human embryo. This cell line can be used in industrial biotechnology and toxicology research. It has applications in efficacy testing and viruscide testing.

What is SEAP assay?

The SEAP Reporter Gene Assay is designed specifically for measuring secreted placental AP (alkaline phosphatase) in conditioned cell culture medium from transfected cells. SEAP is secreted into the culture supernatant where it can be easily sampled and assayed.

What is secreted alkaline phosphatase assay?

Cayman’s Secreted Alkaline Phosphatase Reporter Gene Assay Kit (Luminescence) provides a simple chemiluminescence method for the sensitive quantitation of SEAP in conditioned cell culture medium from transfected cells. The assay can detect SEAP activity in the milliunits/well (mU/ml) range.

What is SEAP gene?