## Does paracetamol absorb UV visible light?

In the UV–vis spectrum, Paracetamol exhibits maximum absorbance (λmax) at 243 nm in methanol, while Aspirin and Caffeine have λmax in proximity to 237 nm and 273 nm, respectively.

What is the absorbance of paracetamol?

243 nm
Results: Paracetamol showed absorption maxima at 243 nm in 0.1N HCland phosphate buffer pH 6.8, while lornoxicam showed absorption maxima at 374 nm in 0.1N HCland phosphate buffer pH 6.8.

### How do you analyze paracetamol?

Paracetamol can be assayed by quantitative estimation using UV Spectrophotometry….

1. The average weight of tablet from each sample was determined by weighing ten tablets and dividing the result by ten.
2. Two tablets were then crushed using a clean pestle and mortar (i.e. from each sample).

What is the wavelength of paracetamol?

Second method is absorbance ratio in which wavelengths selected were 265.6 nm as isoabsorptive point and 275.6 nm as λmax of paracetamol.

## How is UV specific absorbance calculated?

A(l percent, 1 cm) =A/cl, where c is the concentration of the absorbing substance expressed as percentage w/v and I is the thickness of the absorbing layer in cm. The value of A (1 percent, 1 cm) at a particular wavelength in a given solvent is a property of the absorbing substance.

What is the absorbance of paracetamol measured at 257 nm?

5 µg/ml
Absorbances of the solutio recorded at 257 nm (λmax of Paracetamol) nm (Iso-absorptive point). 5 µg/ml.

### What is the suitable wavelength for detection of related substances in paracetamol?

The suitable wavelength for quantitative difference spectrophotometric measurements of paracetamol is at 268 nm (λmax).

What is UV in pharmaceutical analysis?

UV imaging provides spatially and temporally resolved absorbance measurements, which are highly useful in pharmaceutical analysis. Commercial UV imaging instrumentation was originally developed as a detector for separation sciences, but the main use is in the area of in vitro dissolution and release testing studies.

## How do you calculate UV?

How to Calculate UV-C Dose on a Surface

1. To get accurate dose data you need to start with a UV-C detector.
2. UV Dose = UV Intensity (μW/cm² ) x Exposure Time (seconds)
3. Exposure time is how long the UV light is ON and delivering UV to a surface.
4. This value can be compared to the table below for various microorganisms.