Does vectashield have DAPI?
Does vectashield have DAPI?
Description. VECTASHIELD Antifade Mounting Medium with DAPI is a unique, stable formula for preserving fluorescence. This medium inhibits rapid photobleaching of fluorescent proteins and fluorescent dyes.
Does vectashield need to be refrigerated?
The original VECTASHIELD Mounting Medium does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 4 °C, protected from light.
How long does vectashield take to dry?
about 4 – 24 hours
With adequate removal of excess buffer, the VECTASHIELD Vibrance will cure enough by one hour to hold the coverslip in place. Complete curing takes about 4 – 24 hours at room temperature.
What is DAPI mounting medium?
Mounting Medium with DAPI ab104139 (previously called Fluoroshield Mounting Medium With DAPI) is an aqueous anti-fade mounting medium designed to preserve fluorescence when imaging tissues and cell samples. It is fortified with DAPI as a counter-stain for DNA (DAPI also stains RNA).
How much does it cost to mount a medium slide?
use just enough mounting medium to fill the space under the coverslip, as too much will allow the sample to float or move around. Leave 3-5 mm of the slide uncovered at each end.
How long does DAPI staining last?
For long-term storage the stock solution can be aliquoted and stored at =–20°C. For short-term storage the solution can be kept at 2–6°C, protected from light. When handled properly, DAPI solutions are stable for at least six months.
Is product h-1000 (Vectashield) suitable for storm and Sim?
Of the many variations of super resolution microscopy (SRM) currently being used, investigators have found the properties of product H-1000 (VECTASHIELD), to be advantageous in stochastic optical reconstruction microscopy (STORM) and structured illumination microscopy (SIM).
What is the refractive index of Vectashield mountings?
Mounted slides should be stored in the dark at 4 degrees C. VECTASHIELD Mounting Medium is compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. The refractive index is 1.45. DAPI is a counterstain that binds to nuclei and causes them to fluoresce with a blue color.
What mounting mediums are compatible with Vectashield products?
VECTASHIELD Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. For more information about the compatibility of VECTASHIELD products, please consult the VECTASHIELD®Mounting Medium Compatibility table.
How do I store my Vectashield mounting medium?
The original VECTASHIELD Mounting Medium does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 4 degrees C, protected from light.