How do you extract bromelain?

The marketable bromelain is mostly extracted from the stem of pineapple through centrifugation, ultrafiltration, lyophilization (Corzo et al. 2011. Pineapple fruit bromelain affinity to different protein substrates.

How do you isolate bromelain from pineapple?

Results demonstrated that it was possible to purify bromelain extracted from the pineapple peel using ammonium sulfate precipitation (40-80% saturation), followed by desalting, freeze-dry and chromatography on DEAE-Sepharose, and the enzyme could be recovered active after all the steps performed.

Does alcohol denature bromelain?

The results showed that bromelain was recovered by using ethanol at concentrations of 30% and 70%, in which a purification factor of 2.28 fold was achieved, and yielded more than 98% of the total enzymatic activity. This enzyme proved to be susceptible to denaturation after the lyophilization process.

How do you extract a pineapple?


  1. Place the 1 oz of pineapple into the jar. (Or until the jar is about 2/3 of the way full.)
  2. Pour in the rum or alcohol of choice.
  3. Switch out the fruit when it loses color (about 4-6 weeks.)
  4. The extract is done once it’s reached the pineapple flavor you’re wanting! (Usually 8 weeks until completion.)

At what temperature does bromelain denature?

8 minutes at 80 °C (176 °F) is sufficient to almost completely inactivate the enzyme.

Where is bromelain found in pineapple?

Bromelain is a group of enzymes found in the fruit and stem of the pineapple plant. Pineapple is native to the Americas but is now grown throughout the world in tropical and subtropical regions. Historically, natives of Central and South America used pineapple for a variety of ailments, such as digestive disorders.

At what pH does bromelain denature?

Bromelain is an enzyme that breaks down proteins by cleaving some of the bonds between amino acids. Its activity is maximal at pH 4.5. Your hypothesis would have been—at a pH other than 4, the digestion of gelatin will be reduced.

Where is bromelain located in the pineapple?

Background. Bromelain is a group of enzymes found in the fruit and stem of the pineapple plant.

Does freezing destroy bromelain?

No. Freezing does not destroy bromelain or any other enzymes present in pineapple. Freezing does, however, kill the enzymes’ ability to remain active once defrosted.

What is the pH of bromelain?

The optimum temperature and pH for crude bromelain was 50 °C and pH 7.0, respectively, in which was observed the better enzymatic activity. On the other hand, optimum temperature and pH for purified bromelain was 60 °C and pH 8.0, respectively, in which was observed the better enzymatic activity.

What can I do with pineapple waste?

Fermentation of pineapple juice yields wine and upon further fermentation yielding vinegar. Also, pineapple waste can be used for animal feed (pellets and animal feed block), production of paper, and fertilizers because of the high-fiber and cellulose content.