How do you extract DNA phage?

Traditional extraction methods include precipitation of phages, ultra-centrifugation, the use of hazardous solutions, e.g., chloroform, phenol [3], and are time consuming, e.g., the protocol described in Sambrook takes two days [4].

What is Kit Method for DNA extraction?

The DNA Extraction Kit provides a simple, nontoxic method for efficiently isolating high-molecular-weight DNA from tissue, whole blood and cultured cells. Depending on the starting material, the entire extraction takes only two to three hours to complete and does not require phenol or chloroform.

How are phages isolated?

Phages are purified by removing, picking off, a well isolated plaque using either a Pasteur pipette or more crudely, but just as effectively, a wire loop. Using a sterile Pasteur pipette the area around the plaque is stabbed and pieces of soft area are ‘sucked’ into the pipette.

Does bacteriophage have DNA or RNA?

Bacteriophage have either DNA or RNA as their genetic material, in either circular or linear configuration, as a single- or a double-stranded molecule.

How do miniprep kits work?

Aka ALKALINE LYSIS, “minipreps” are experiments in which we separate and purify the plasmid DNA we put into bacteria from all the stuff that was already in the bacteria. You can’t just break the cells open (lyse them) & pull out all the DNA because the bacteria has its own DNA you aren’t interested in.

Is DNA extraction expensive?

Material costs for the three DNA extraction methods varied significantly (Table 1). Cost per sample (US$) was $3.04 for DNeasy™ kits, $0.99 for the DNAzol® Reagent method, and $0.39 for the PEX/CTAB extraction.

Why is salt used in DNA extraction?

WHY SALT WATER? Your DNA’s sugar phosphate backbone is charged. By adding salt, we help neutralize the DNA charge and make the molecule less hydrophilic, meaning it becomes less soluble in water. The salt also helps to remove proteins that are bound to the DNA and to keep the proteins dissolved in the water.