How much DNA do you get from a miniprep?

The GeneJET Plasmid Miniprep Kit recovers up to 20 µg of high copy plasmid DNA per isolation procedure utilizing a silica-based membrane within the spin column.

How much DNA is in a MaxiPrep?

Plasmid MaxiPrep Kit Benefits High recovery Up to 1 mg of plasmid DNA can be purified from 100 mL of bacterial culture.

How many lbs is MaxiPrep?

To expand the starter culture to a larger culture, dilute it 1/500 to 1/1000 in LB medium containing antibiotic. For example, to make a 150 mL culture for a Maxiprep, add between 150 and 300 µL of the starter culture to 150 mL of LB medium containing antibiotic and grow for 12-16 hrs with shaking.

What is Qiagen DNeasy?

The DNeasy membrane combines the binding properties of a silica-based membrane with simple microspin technology or with the QIAGEN 96-Well-Plate Centrifugation System. DNA adsorbs to the DNeasy membrane in the presence of high concentrations of chaotropic salt, which remove water from hydrated molecules in solution.

What is the Qiagen product number for buffer MP?

It is a proprietary component of the QIAprep Spin Miniprep Kit. However, buffer N3 can be purchased separately. The catalogue number is 19064. The item is listed in the QIAGEN Product List online. How do I prepare Buffer MP? Dissolve 3.3 g citric acid monohydrate in 3 ml high-purity water at room temperature (21°C) in a 10 ml tube.

What’s new in QIAGEN’s plasmid preparation kits?

The maximum culture volumes recommended for QIAGEN’s plasmid preparation kits still apply, and should be strictly followed. LyseBlue reagent now allows the user to monitor potential problems (insufficient bacterial cell resuspension and lysis as a consequence of overloading) early in the plasmid preparation process.

What is a qiaquick PCR purification kit?

QIAquick PCR Purification Kit. The QIAquick PCR Purification Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of PCR products >100 bp. DNA of up to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μl.

How much RNA can I isolate with the QIAGEN plasmid MIDI kit?

PDF(59KB) This protocol is designed for isolation of up to 200 μg RNA from 150 mg plant tissue or up to 1 mg RNA from 600 mg plant tissue and is for use with QIAGEN-tip 100 or QIAGEN-tip 500, respectively. Isolation of endotoxin-free plasmid DNA using the QIAGEN® Plasmid Midi Kit – (EN)