What are 2 disadvantages of tissue culture in bananas?

Disadvantages of Tissue Culture Propagation of Banana

  • The tissue culture experiment is expensive because of the costly equipment and reagents used in the experiments.
  • The procedure is complex.
  • The experiment or procedure of culturing is required to be performed by an expert or highly trained person.

Which part of banana is used for tissue culture?

Different stages of banana keikis (peepers, sword, or maiden suckers) about 1–3 ft (40–100 cm) tall that are free of BBTV symptoms can be collected for tissue culture. Separate the desired keiki from the main stem without cracking the corm of the keiki.

How do you make banana tissue culture?

Tissue Culture Banana Plants Tissue culture means cloning and micro-propagation of tissues of the selected Elite plants and daughter suckers. The process consists of five important steps: Initiation, Multiplication, Shooting & rooting, Primary Hardening in green houses and Secondary Hardening in shade houses.

How deep should a banana hole be?

Bananas require a lot of water, but are prone to rotting if the water does not drain adequately. To test drainage, dig a hole 0.3m (1 ft.) deep, fill with water, and allow to drain. Refill once empty, then measure how much water is left after 1 hour.

Why is tissue culture better than cuttings?

Advantages of Tissue Culture The new plantlets can be grown in a short amount of time. Only a small amount of initial plant tissue is required. The new plantlets and plants are more likely to be free of viruses and diseases. The process is not dependant on the seasons and can be done throughout the year.

What are the limitations of tissue culture?


  • Tissue Culture can require more labor and cost more money.
  • Maintaining the sterile aseptic condition is the most difficult part of cell culture.
  • It may be vulnerable to many environmental factors since they have same genetic material.

What is the explant of banana?

For rapid in vitro multiplication of banana, shoot tips from young suckers of 40-100 cm height are most commonly used as explants. From the selected sucker a cube of tissue of about 1-2 cm³ containing the apical meristem is excised.

Which part of banana is used for propagation?

The banana has a reduced underground stem, called the rhizome, which bears several buds. Each of these buds sprouts and forms its own pseudostem and a new bulbous rhizome. These daughter plants are called suckers. Banana is mostly propagated by rhizomes and suckers viz.

What is the procedure of tissue culture?

Plant tissue culture is a practice of propagating plants under sterile conditions, often to produce clones of a plant. Process of tissue culture: Plants are grown in test tubes. A small part of the plant is allowed to grow on a nutrient medium containing all nutrients and hormones necessary for the plant growth.