What are U937 cells used for?
What are U937 cells used for?
U-937 cells are a model cell line used in biomedical research. They were isolated from the histiocytic lymphoma of a 37-year-old male patient and are used to study the behaviour and differentiation of monocytes.
What are the uses of cell lines?
Cell lines have revolutionized scientific research and are being used in vaccine production, testing drug metabolism and cytotoxicity, antibody production, study of gene function, generation of artificial tissues (e.g., artificial skin) and synthesis of biological compounds e.g., therapeutic proteins.
What type of cells are U937?
U937 is a pro-monocytic, human myeloid leukaemia cell line and was isolated from the histiocytic lymphoma of a 37 year old male (Sundstrom and Nilsson 1976). This cell line exhibits many characteristics of monocytes and is easy to use.
What is a research cell line?
A cell line is a collection of cells originating from one cell. Cell lines are typically kept in a growth medium in tubes, flasks, or dishes – called in vitro cell culture. Here, they can continue to divide indefinitely.
Is U937 an AML cell line?
The U937 cell line was originally established from the pleural effusion of a patient with histiocytic lymphoma and is usually considered to be an AML cell line (10, 11).
What are cell lines?
Cell lines are cultures of animal cells that can be propagated repeatedly and sometimes indefinitely. They arise from primary cell cultures. Primary cultures are initiated directly from the cells, tissues, or organs of animals and are typically used in experiments within a few days.
What is cell line in microbiology?
The term cell line refers to the propagation of culture after the first subculture. In other words, once the primary culture is sub-cultured, it becomes a cell line. A given cell line contains several cell lineages of either similar or distinct phenotypes.
How do you differentiate U937 cells?
U937 Cell Differentiation by Phorbol 12-Myristate 13-Acetate U937 pro-monocytes differentiate into mature monocytes or macrophages upon PMA-treatment, and it has been reported that differentiation is typically observed after 36–48 h; however, it can also depend on the concentration of inducers.
U937 cells were isolated from a histiocytic lymphoma of a 37-year-old male patient in 1974. Because they are one of just a few cell lines available that express many monocytic characteristics, they are often used to study the behavior and differentiation of monocytes.
Is the human monocytic cell line U937 a suitable model for mycobacterial antigens?
In summary, this study has demonstrated that the human monocytic cell line, U937, is a suitable in vitro model for HLA class I-restricted presentation of mycobacterial antigens to human cytolytic T cells.
What is the viability of a U937 line?
I recently started a U937 line. On the day I thawed the vial of cells they were at 50% viability (by trypan blue exclusion). When I checked them the next day they were at 10% viability.
How to dilute U937 cells for macrophage survival assay?
I am working with the U937 cell line, which I wish to use for macrophage survival assays. For my protocol I dilute my cells to 0. 5×106 cells per ml (or well) and add PMA at a concentration of 100ng/ml for 24 hours.