What are used for semen extender?

Egg yolk is the primary non-penetrate substance used in extenders to dilute semen and protect sperm from freeze shock during the chilling process. Egg yolk-based extender is commonly used in chilled, frozen semen, or both.

How can I extend my semen stallion?

Skim-milk extender appears to be the medium best suited for the preservation of stallion semen during cooling and storage. When used immediately, semen is usually extended 1:1 (v:v), but for transport, concentrations of 25 to 100 x 10(6) spermatozoa/mL are recommended.

How do you mix a water extender solution?

The solution must be stored in the refrigerator and used within 3 weeks of preparation or until the sperm fails to initiate motility when diluted again with water. Use the extender by mixing one part semen with two to three parts extender.

How long does sperm extender sperm live?

Depending on the extender chosen, extended boar semen can be stored for up to 6 days with no appreciable loss in fertility potential. Most recently, semen extenders purported to maintain sperm viability for up to 10 days are being marketed; field fertility trials, however, are equivocal at this time.

Can sperm survive in coconut oil?

It might sound slightly nutty, but natural oils like canola, almond, olive, or coconut oil all make excellent lubricants. Each of these are easily and naturally absorbed by the body, and are all safe on sperm.

What is the use of diluent and extenders?

Semen Extender or diluent is a chemical medium used for preservation, extension and protection of sperm cells against various shocks during processing, storage and transportation used for artificial insemination.

How do you use Equitainer?

Equitainer

  1. Freeze coolant cans for at least 24 hr.
  2. Pack semen in at least 2 plastic bags.
  3. Roll the semen and ballast bags together and place in the specimen container.
  4. Put the coolant cans in the Equitainer (in plastic bag).
  5. Place the specimen container in the isothermalizer.

What is Tris based extender?

Tris based extenders have been used for storage of semen collected from bull, boar, goat, and ram (Salamon and Maxwell, 2000). The concentrations of Tris ranging from 10 mM to 50 mM have little or no protective effects on motility and metabolism of ram spermatozoa.

Can you overnight sperm?

Sperm will not live more than a few hours without a special sperm extender. The real secret to shipping sperm is the use of TYB, or Test Yolk Buffer. TYB is a sperm extender that feeds the sperm a nutrient rich egg yolk that is actually an extraction from chicken eggs.

Is Vaseline sperm-friendly?

Saliva is not a sperm-friendly medium, nor is water. Anything petroleum-based, such as Vaseline, should be avoided. Most other, artificial lubricants (such as KY Jelly, Astroglide, Femglide) have been found in several studies to have a negative effect on spermatozoa.

What is the best lubricant for getting pregnant?

A quick look at the best fertility-friendly lubricants

  • Most popular: Fairhaven Health BabyDance Fertility Lubricant.
  • Most convenient: Conceive Plus Fertility Lubricant.
  • Expert’s choice: Pre-Seed Fertility Lubricant.
  • Best all-natural lube: Good Clean Love Biogenesis Fertility Lubricant.
  • Best reviews: Natalist The Lube.

What is the pH value of sperm?

from 7.2 to 8.0
Abstract. Objective: The World Health Organization laboratory manual, last revised in 1992, states that the normal pH of semen ranges from 7.2 to 8.0.

How do semen extenders preserve sperm?

Semen extenders preserve sperm by stabilizing its properties, including sperm morphology, motility, and viability and membrane, acrosomal, and DNA integrity. Therefore, semen extenders must provide a favorable pH, adenosine triphosphate, anti-cooling and anti-freeze shock, and antioxidant activity to improve semen quality for fertilization.

How much penicillin do you put in a semen extender?

Texas workers suggest that either a combination of potassium penicillin G (1000 U/mL) and amikacin sulfate (1 mg/mL) or timentin (1 mg/mL) in a milk-based semen extender may optimize longevity of sperm motility while providing good broad-spectrum antibacterial activity.

What is the best semen extender for horses?

Most commonly used equine semen extenders are milk based (Table 12-1). Some milk-based extenders are available commercially (Table 12-2). Addition of appropriate antibiotics to semen extenders aids elimination of bacteria, which invariably contaminate the semen sample during its collection.

What egg yolks can be used in semen extenders?

Poultry egg yolk can be used in the semen extenders; however, some studies show the possibility to use different egg yolks of animals, such as quail [81], turkey [82], duck [83], pigeon [82], goose [84], and ostrich [85].