What does HaCaT stand for?

Definition. HaCaT. Cultured Human Keratinocyte (cells)

What is a HaCaT cell line?

HaCaT is a spontaneously transformed aneuploid immortal keratinocyte cell line from adult human skin, widely used in scientific research. HaCaT cells are utilized for their high capacity to differentiate and proliferate in vitro.

What triggers keratinocyte differentiation?

Calcium is the major regulator of keratinocyte differentiation in vivo and in vitro. A calcium gradient within the epidermis promotes the sequential differentiation of keratinocytes as they traverse the different layers of the epidermis to form the permeability barrier of the stratum corneum.

How often are keratinocytes replaced?

every 40–56 days
In humans, it is estimated that keratinocytes turn over from stem cells to desquamation every 40–56 days, whereas in mice the estimated turnover time is 8–10 days.

How do you detach a HaCaT cell?

Splitting HaCaT Cells

  1. Remove media from flask and add 10ml PBS to rinse cells then remove;
  2. Add 2-3ml trypsin and leave until all cells have rounded up and begun to detach (for ~10min) in 37oC/5% CO2 incubator;

What are CaSki cells?

CaSki cells are a human papillomavirus type 16 (HPV-16)-positive cell line that serves as a model for the study of advanced cervical carcinoma. Calcium is an important regulator of normal ectocervical epithelial cell differentiation.

What cells cause psoriasis?

Since then, it has been widely recognized that the interaction of T cells and DCs creates a ‘type 1’ inflammatory environment by secreting large amounts of Th1 type cytokines, leading to the development of psoriasis.

What happens if keratinocytes are damaged?

Upon damage the keratinocytes initiate a pro-inflammatory signaling cascade that leads to the activation of resident immune cells. Simultaneously, the tissue mediates and supports immune-suppressive functions to contain inflammation locally.

What is the difference between keratinocytes and melanocytes?

Keratinocytes are the most abundant cells in the epidermis. They are responsible for the production of keratin that serves as the mechanical barrier for damage. Melanocytes are responsible for the production of melanin that provides the color to the skin.