What is a positive nitrazine test?

Nitrazine Test This test involves putting a drop of fluid obtained from the vagina onto paper strips containing Nitrazine dye. The strips change color depending on the pH of the fluid. The strips will turn blue if the pH is greater than 6.0. A blue strip means it’s more likely the membranes have ruptured.

What color does nitrazine paper turn with amniotic fluid?

Because amniotic fluid is neutral (pH 7.0) or slightly alkaline (pH 7.25), it will change the yellow color of Nitrazine Paper. The pH values of blood, vaginal mucus, and certain secretions form vaginal infections are also Page 5 Procedures 5 Sterile Speculum Exam Policy and Procedure (con’t) alkaline.

What is nitrazine positive fluid?

Positive: Ruptured Membranes (pH 6.5-7.5) Clinical Significance. Nitrazine (Phenaphthazine) paper is used to measure the vaginal pH of expectant mothers to determine if the amniotic membrane has ruptured. The pH is used to determine if the fluid present represents amniotic fluid or vaginal fluid.

Which of the following can cause a false positive result for a nitrazine test?

Nitrazine test may also lead to false positive or negative results due to probable contamination by alkaline urine, semen, blood, meconium, vaginitis, cervicitis and using antibiotics. Fern test has also 13-30% false negative and 5-30% false positive results (13, 14).

What Colour is amniotic fluid?

Normal amniotic fluid is clear or tinted yellow. Fluid that looks green or brown usually means that the baby has passed the first bowel movement (meconium) while in the womb.

How do you use Nitrazine paper?

Quality Control Procedure Wearing gloves, tear off a strip of Nitrazine paper from the roll 3. Dispense a drop of the pH 5.0 buffer solution onto the strip of pH paper. 4. Immediately match the color of the pH paper with the closest color on the Color Chart supplied with the Nitrazine paper dispenser.

How do you use Nitrazine paper for amniotic fluid?

Bring the strip of Nitrazine paper into contact with the specimen, either by dipping the paper into the pool of suspected amniotic fluid or by touching the paper to the exam speculum.

How do you use nitrazine paper?

Is Nitrazine paper the same as pH paper?

Nitrazine or phenaphthazine is a pH indicator dye often used in medicine. More sensitive than litmus, nitrazine indicates pH in the range of 4.5 to 7.5. Nitrazine is usually used as the disodium salt….Use.

Nitrazine (pH indicator)
below pH 4.5 above pH 7.5
4.5 7.5

What is the color of water breaking?

The color of the fluid when your water breaks is usually clear or pale yellow, and the fluid has no smell.

Can amniotic fluid be pink?

Amniotic fluid should be clear and odorless. It may also be pink-tinged, green or another color or foul smelling.

How do you test Nitrazine paper?


  1. Add one drop of buffer to Nitrazine test paper.
  2. Compare color to the paper dispenser’s pH scale. The pH must be within 0.5 of the designated pH to be acceptable.
  3. Document all results on the Control Sheet. If controls are out of range do not report any patient results until corrective action has been taken: