What is a UV crosslinker used for?

The Crosslinkers are designed to measure and control the ultraviolet (UV) radiation within the exposure chamber. A unique UV sensor continually measures the UV energy and automatically adjusts to variations in UV intensity that occurs as the UV tubes age.

What is UV Crosslink?

UV cross-linking assay is a standard method used to detect protein-RNA interaction. This method takes advantage of UV irradiation to trigger the formation of the covalently bonded RNP (ribonucleoprotein) complex that is more stable and makes it possible to be isolated in the denaturing conditions.

What does a Stratalinker do?

The Stratalinker UV crosslinker can be used to link nucleic acids to membranes after Southern blotting, Northern blotting, slot blotting, dot blotting, colony lifts, or plaque lifts.

Which is the cross linking agent used in in situ hybridization process?

The use of UV light as a cross-linking agent for cells and tissue sections in in situ hybridization. DNA Cell Biol.

How do you study DNA protein interactions?

The DNA electrophoretic mobility shift assay (EMSA) is used to study proteins binding to known DNA oligonucleotide probes and can be used to assess the degree of affinity or specificity of the interaction.

How does Cish work?

Chromogenic in-situ hybridization (CISH) is a relatively new method for determination of gene amplification using a peroxidase-based chromogenic reaction, which can be viewed using a conventional bright field microscope. Like FISH, CISH determines the actual degree of HER2 gene amplification.

What is the difference between FISH and ish?

In Situ Hybridization (ISH) and Fluorescence in Situ Hybridization (FISH) In situ Hybridization (ISH) is a method that allows to localize and detect nucleic acid sequences within structurally intact cells or morphologically preserved tissues sections.

Which technique is used for DNA bond detection?

Quantitative PCR (qPCR) technique allows the amount of target DNA sequence to be quantified.