What is an Uninoculated medium?

(of a culture medium) not having been inoculated with organisms, esp bacteria.

Were the uninoculated controls positive or negative controls?

Were the uninoculated controls in this lab positive or negative, and what purpose did they serve? The controls were negative, and served as color comparison for experimental tubes as well as verification for media sterility.

What information does the Uninoculated tube provide?

b) What information is provided by the uninoculated control? b) It provides a baseline color (green) for “no change’ in the medium. This allows detection of slight color changes in the experimental tubes that might not be apparent without comparison to the original color. It also verifies the sterility of the medium.

Is TSA selective or differential?

Tryptic Soy Agar (TSA): a general purpose, non-selective, non-differential, supportive medium that supports growth of all microorganisms that do not require special nutrients.

What is the purpose of uninoculated control tubes?

Two uninoculated control tubes are needed to show the results of the medium in both aerobic and anaerobic environments. It is used to show that the medium remains green under both conditions, showing it is sterile and also as a color comparison. 4.

What is the purpose of the uninoculated control tubes used in this test?

Why are TSA plates used?

Tryptic Soy Agar (TSA) is a general purpose plating medium used for the isolation, cultivation, and maintenance of a variety of fastidious and non- fastidious microorganisms. Leavitt et al. demonstrated the versatility of TSA by cultivating both aerobic and anaerobic microbes using TSA.

Were the uninoculated controls positive or negative controls and what purpose did they serve?

Why do you shake the VP tube?

A false negative on the VP test is likely due to a lack of sensitivity- there is not enough acetoin to react with the test reagents. Why were you told to shake the VP tubes after the reagents were added? The indicator reaction requires oxygen to oxidize acetoin to diacetyl.