What is DPPH free radical scavenging activity?

What is DPPH free radical scavenging activity?

DPPH free radical scavenging is an accepted mechanism for screening the antioxidant activity of plant extracts. In the DPPH assay, violet color DPPH solution is reduced to yellow colored product, diphenylpicryl hydrazine, by the addition of the extract in a concentration dependent manner.

What is the DPPH method?

DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free radical method is an antioxidant assay based on electron-transfer that produces a violet solution in ethanol (10). This free radical, stable at room temperature, is reduced in the presence of an antioxidant molecule, giving rise to colorless ethanol solution.

Why is DPPH a stable free radical?

The molecule of 1,1-diphenyl-2-picryl- hydrazyl (α,α-diphenyl-β-picrylhydrazyl; DPPH: 1) is characterised as a stable free radical by virtue of the delocalisation of the spare electron over the molecule as a whole, so that the molecules do not dimerise, as would be the case with most other free radicals.

What is DPPH inhibition?

2, 2–diphenyl–1–picrylhydrazyl (DPPH) inhibition was used to evaluate the antioxidant activity of CPS. Response surface methodology (RSM) was used to investigate the effects of PEF treatment parameters on antioxidant activity of CPS.

How do you do DPPH activities?

1. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) 0.24 mg/mL :
2. 1.1.1 Weigh 0.024 g of DPPH in a piece of aluminum foil;
3. 1.1.2 Add 50 mL of absolute ethanol to the beaker to dissolve the salt;
4. 1.1.3 Transfer your solution to a 100 mL volumetric flask;
5. 1.1.4 Using absolute ethanol, complete the solution’s volume to reach 100 mL ;

How is DPPH value calculated?

In DPPH free radical scavenging method, IC50 (Half maximal Inhibitory Concentration) value is the concentration of the sample that could scavenge 50% of DPPH free radical. DPPH inhibition (%)={(A0 –A1)/A0}×100 where A0 is the absorbance of control and A1 is the absorbance of test.

How do you do DPPH assay?

What is the structure of DPPH?

DPPH is a common abbreviation for the organic chemical compound 2,2-diphenyl-1-picrylhydrazyl. It is a dark-colored crystalline powder composed of stable free radical molecules….DPPH.