What is Lambda HindIII marker?

Description. The Lambda DNA is completely digested with HindIII to yield bands ranging from 0.125 kb to 23 kb, suitable for use as molecular weight standards for agarose gels. The marker is composed to 8 purified individual DNA fragments (in base pairs): 23130*, 9416, 6557, 4361*, 2322, 2027, 564 and 125.

What is Lambda HindIII DNA marker and what is its function?

Thermo Scientific Lambda DNA/HindIII Marker, ready-to-use is recommended for sizing of linear double-stranded large DNA on agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme(s) and purified and dissolved in storage buffer.

What are DNA size markers?

DNA and RNA size markers contain a mixture of DNA (or RNA) fragments of known length, making them suitable for estimating the fragment length of concurrently run samples. Markers and ladders stain well with ethidium bromide and other common nucleic acid stains for visualization after gel electrophoresis.

What is lambda DNA?

Lambda DNA, a linear, double-stranded phage DNA containing 12 bp single-stranded complementary 5′-ends, is derived from an Escherichia coli bacteriophage (Bacteriophage lambda cI857 Sam7).

How do I choose a DNA marker?

Size range The most important factor in choosing a DNA ladder is the expected size of the DNA bands in your experiment. You want to make sure that the range of the DNA ladder, from its smallest to its largest fragment, includes the expected size of the DNA fragments you are testing (Figure 1).

What is protein marker?

A protein marker (also called a protein molecular weight marker, a protein MW marker, or a protein ladder) is used to estimate the size of proteins resolved by gel electrophoresis. All markers are optimized for use with LI-COR imaging systems but can be used with other imagers.

What is lambda infection?

Enterobacteria phage λ (lambda phage, coliphage λ, officially Escherichia virus Lambda) is a bacterial virus, or bacteriophage, that infects the bacterial species Escherichia coli (E. coli). It was discovered by Esther Lederberg in 1950.

What does lambda mean in biology?

a naturally occurring double-stranded bacteriophage with a linear genome of about 50,000 base pairs.

Thermo Scientific Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme(s) and purified and dissolved in storage buffer.

What is Lambda ladder?

The lambda ladder consists of suc- cessively larger concatemers of λ cl857 Sam7. These DNA size standards may be used as size markers for CHEF, FIGE, and other pulsed field electrophoresis gels (Figure 1). Quantity. Five agarose inserts (25–40 lanes).

What is lambda ladder?

What is λ DNA?

What is the size of the lambda genome in kilobases?

The DNA of Bacteriophage λ is approximately 48,514 base pairs or 48.514 kilobase pairs in length while the human genome is approximately 3 billion base pairs.

What is lambda repressor?

The lambda repressor is a self assembling dimer also known as the cI protein. It binds DNA in the helix-turn-helix binding motif. It regulates the transcription of the cI protein and the Cro protein. The life cycle of lambda phages is controlled by cI and Cro proteins.

What are lambda vectors?

Bacteriophage lambda has been in use as a cloning vector for over 25 years, and has been used extensively as an expression vector. The efficiency of packaging and infection, and the simplicity of plaque screening are advantages of lambda as a cloning vector.

What is lambda PCR?

Description: Lambda DNA is recommended as template in positive control PCRs, as substrate in restriction enzymes research and for testing of restriction endonucleases activity. The double stranded DNA is isolated from bacteriophage lambda (cl857 ind1 Sam7). Double stranded DNA with 48,502 base pairs.

Does HindIII create sticky ends?

HindIII restrictions process results in formation of overhanging palindromic sticky ends.

What is HindIII used for?

Their primary function is to protect the host genome against invasion by foreign DNA, primarily bacteriophage DNA. There is also evidence that suggests the restriction enzymes may act alongside modification enzymes as selfish elements, or may be involved in genetic recombination and transposition.

What is lambda in gel electrophoresis?

Lambda DNA (48,502 bp) may be used as a molecular weight size marker during nucleic acid gel analysis following digestion with a restriction enzyme (such as HindIII). Lambda DNA can also be used as a substrate in restriction enzyme activity assays.