What is Proteinase K solution?

Proteinase K Solution is a highly active serine protease that exhibits broad cleavage specificity. Isolated from Tritirachium album is used for protease digestion during DNA and RNA preparation.

What can be used instead of Proteinase K?

Nagarse was thus comparable to proteinase K for use in biochemical experiments. The principal advantage of Nagarse is that it is inexpensive, unlike proteinase K, and our findings indicated that Nagarse is very useful as a substitute for proteinase K for the DNA study.

How do you make a Proteinase K solution?

To ensure maximum Proteinase K activity, use water that has been treated with 0.1% DEPC (DEPC, GoldBio Catalog # D-340 [CAS 1609-47-8, mw. = 162.14 g/mol]) to make the buffer. Add 1 ml of DEPC to 1 L of H2O, shake vigorously, and let incubate for 12 hours at 37°C to inactivate any RNases.

How do you use proteinase K?

Proteinase K is used mostly in DNA and RNA extraction protocols. You’ll often find the proteinase K step within the lysis section of the protocol. For example, in the nucleic acid extraction protocol, proteinase K is added to cell lysate and then an incubation period follows to ensure a complete digestion.

Is proteinase K necessary for RNA extraction?

Proteinase K is critical because it digests proteins eliminating contamination from nucleic acid preparations, in addition to inactivating the nucleases that could degrade DNA or RNA during purification.

How do you dissolve proteinase K powder?

Reconstitute Proteinase K Dissolve the Proteinase K: • Dissolve in 10 mM Tris HCl, pH 7.5, 20 mM calcium chloride, and 50% glycerol, then store at –20°C. Dissolve in 50 mM Tris HCl (pH 8.0), 1–5 mM calcium acetate, then store at 2°C–8°C.

What is QIAGEN proteinase K?

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times.

What is proteinase K solution made of?

Proteinase K, produced by the fungus Tritirachium album Limber, is a serine protease that exhibits broad cleavage activity. Proteinase K (PK) Solution is supplied at a concentration of 20mg/ml in 10mM Tris-HCl (pH 7.5), 1mM calcium chloride, 50% glycerol.

What is the pH of QIAGEN protease?

* pH 8.0, 50°C, 1.25 µg/ml protease, 15 min incubation. † Buffer G2 used in QIAGEN Genomic-tip procedures for DNA isolation from blood, cell cultures, and tissue.

What is the function of Proteinase K in fungi?

Proteinase K, produced by the fungus Tritirachium album Limber, is a serine protease that exhibits broad cleavage activity. It cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids and is useful for general digestion of protein in biological samples.