What is the Km apparent?

Kmapp is used when the km value of an enzyme is measured in the presence of an inhibitor or at conditions of higher substrate concentration which itself can serve as inhibitor to the enzyme.

What does the Km value tell you about a protein?

It indicates the affinity of an enzyme for a given substrate: the lower the KM value, the higher the affinity of the enzyme for the substrate.

What is a Km value?

Km value is equal to the substrate concentration at which half of the enzyme active sites are saturated with the substrate. It tells about the affinity of enzymes for their substrate. Km is the concentration of substrate at which half of the Vmax is attained.

What does high Km mean in enzyme kinetics?

a low affinity
An enzyme with a high Km has a low affinity for its substrate, and requires a greater concentration of substrate to achieve Vmax.”

What does high Km mean?

low affinity

What does Michaelis constant tell us?

Km is the Michaelis-Menten constant which shows the concentration of the substrate when the reaction velocity is equal to one half of the maximal velocity for the reaction. It can also be thought of as a measure of how well a substrate complexes with a given enzyme, otherwise known as its binding affinity.

How do you calculate Km value?

From the graph find the maximum velocity and half it i.e. Vmax/2. Draw a horizontal line from this point till you find the point on the graph that corresponds to it and read off the substrate concentration at that point. This will give the value of Km.

What is Vmax in Michaelis Menten?

Vmax is the reaction rate when the enzyme is fully saturated by substrate, indicating that all the binding sites are being constantly reoccupied.

Is a lower Km better?

The less fuel you need to reach “normal speed”, the more efficient your car is. That’s sort of how enzymes work. The less substrate they need to reach half of their maximum speed, the more efficient they are. So if the Km is low, you have a really efficient enzyme.